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Analysis and application of ATP7B gene mutations in 35 patients with hepatolenticular degeneration
Authors: Y.N. Zong and X.D. Kong
We investigated the genetic mutations involved in Wilson’s disease to improve prenatal genetic diagnosis and presymptomatic diagnosis. The polymerase chain reaction (PCR) was used to amplify the exons and exon-intron boundaries of the ATP7B gene in 35 Wilson’s disease pedigrees. The PCR products were further analyzed by Sanger sequencing. Pren.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.25
- DOI:
- 10.4238/2015.December.28.25
Effects of stathmin 1 silencing by siRNA on sensitivity of esophageal cancer cells Eca-109 to paclitaxel
Authors: H.W. Zhu, D. Jiang, Z.Y. Xie, M.H. Zhou, D.Y. Sun and Y.G. Zhao
We investigated the effects of stathmin 1 (STMN1) silencing by small interfering (siRNA) on the sensitivity of esophageal cancer cells Eca-109 to paclitaxel. STMN1 siRNA was transiently transfected into Eca-109 cells. The effects of transfection were detected by quantitative polymerase chain reaction and western blotting. The effects of STMN1 silencing by.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.18
- DOI:
- 10.4238/2015.December.28.18
miRNA-146a induces vascular smooth muscle cell apoptosis in a rat model of coronary heart disease via NF-�ºB pathway
Authors: Z.W. Wu, Y.F. Liu, S. Wang and B. Li
The aim of this study was to investigate the role of miRNA-146a in modulating the function of vascular smooth muscle cells in a rat model of coronary heart disease. Vascular smooth muscle cells were isolated and cultured from the rat coronary heart disease model and normal rats (controls). miRNA-146a levels were measured in vascular smooth muscle cells ob.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.19
- DOI:
- 10.4238/2015.December.28.19
Male carriers of balanced reciprocal translocations in Northeast China: sperm count, reproductive performance, and genetic counseling
Authors: H.G. Zhang, R.X. Wang, L.L. Li, W.T. Sun, H.Y. Zhang and R.Z. Liu
Balanced chromosomal translocations in men can cause failure of spermatogenesis owing to meiotic impairment. Male carriers may exhibit normozoospermia, although clinical manifestations can include oligozoospermia or azoospermia, oligozoospermia or normozoospermia. Here, we reported the characteristics of balanced reciprocal translocations in men from nort.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.28
- DOI:
- 10.4238/2015.December.28.28
Molecular tracing of white muscardine in Asian corn borer using inter-simple sequence repeat (ISSR) analysis
Authors: B.J. Hu, L.N. Xu, Z.Y. Zhou, F. Hu, F.G. Luan, X. Chen and Z.Z. Li
Beauveria bassiana is a soil fungus that parasitizes arthropod species, and is used to control the Asian corn borer in Northeast China. In this study, B. bassiana was investigated in Xiaoxian County and Baicheng City, and the results were compared with those of Gongzhuling City, where the fungus was not applied. Using the inter-simple sequence repeat (ISS.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.21
- DOI:
- 10.4238/2015.December.28.21
Association between TAP1 gene polymorphisms and alopecia areata in a Korean population
Authors: H.K. Kim, H. Lee, B.L. Lew, W.Y. Sim, Y.O. Kim, S.W. Lee, S. Lee, I.K. Cho, J.T. Kwon and H.J. Kim
The transporter 1 ATP-binding cassette sub-family B (MDR/TAP) gene (TAP1) is located in the major histocompatibility complex class II region, and forms a heterodimer that plays a key role in endogenous antigen presentation pathways. Investigation of polymorphisms identified in these loci has revealed an association with several autoimmune disorders. Alope.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.31
- DOI:
- 10.4238/2015.December.28.31
Association between PAI-1 polymorphisms and plasma PAI-1 level with sepsis in severely burned patients
Authors: Y.F. Chi, J.K. Chai, Y.M. Yu H.M. Luo, Q.X. Zhang and R. Feng
We investigated the association between plasminogen activator inhibitor-1 (PAI-1) polymorphisms and plasma PAI-1 level with sepsis in severely burned patients. A total of 182 patients with burn areas lager than 30% of the body surface area were enrolled in this study. Peripheral blood samples were obtained from 103 patients with sepsis (sepsis group) and .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.15
- DOI:
- 10.4238/2015.August.21.15
Modified Da Chengqi granules improvement in immune function in early severe acute pancreatitis patients
Authors: D.-L. Jiang, J. Yang, S.-Y. Jiang, F.-L. Yuan, Y.-L. Gu, J.-P. Li and Z.-J. Pei
We investigated the role of modified Da Chengqi granules in improving immune function in early severe acute pancreatitis patients. Early severe acute pancreatitis patients who agreed to receive combined treatment of traditional Chinese and Western medicine were randomly assigned to the experimental or control group. All subjec.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028787
- DOI:
- 10.4238/gmr.15028787
Multiple sequence alignment based on combining genetic algorithm with chaotic sequences
Authors: C. Gao, B. Wang, C.J. Zhou and Q. Zhang
In bioinformatics, sequence alignment is one of the most common problems. Multiple sequence alignment is an NP (nondeterministic polynomial time) problem, which requires further study and exploration. The chaos optimization algorithm is a type of chaos theory, and a procedure for combining the genetic algorithm (GA), which use.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028788
- DOI:
- 10.4238/gmr.15028788
Identification of genes associated with the increased number of four-seed pods in soybean (Glycine max L.) using transcriptome analysis
Authors: Z.Z. Liu, D. Yao, J. Zhang, Z.L. Li, J. Ma, S.Y. Liu, J. Qu, S.Y. Guan, D.D. Wang, L.D. Pan, D. Wang and P.W. Wang
Seed number per pod is an important component of yield traits in soybean (Glycine max L.). In 2010, we identified a natural mutant with an increased number of four-seed pods from a soybean variety named ‘Jinong 18’ (JN18). Subsequent observations indicated that the trait was stably inherited. To identify and understand the function of genes as.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.39
- DOI:
- 10.4238/2015.December.28.39