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Sevoflurane downregulates interleukin-6 and interleukin-8 levels in patients after cardiopulmonary bypass surgery: a meta-analysis
Authors: Q.B. Yu, H.M. Li, L.L. Li, S.Y. Wang and Y.B. Wu
This study aimed to investigate the effect of sevoflurane on serum levels of interleukin (IL)-6 and IL-8 in patients who underwent cardiopulmonary bypass (CPB). The strength of the association between sevoflurane treatment and serum level of IL-6 and IL-8 was determined in patients who underwent CPB by summary standard mean differences (SMDs); 95% confide.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.9
- DOI:
- 10.4238/2015.December.29.9
Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp
Authors: F. Liu and Y.P. Cao
The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroAPantoea sp plasmid. Amino acid sequence analysis indicated that AroAPantoea s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.33
- DOI:
- 10.4238/2015.December.29.33
Assessment of genetic diversity of cucumber cultivars in China based on simple sequence repeats and fruit traits
Authors: Y.T. Yang, Y. Liu, F. Qi, L.L. Xu, X.Z. Li, L.J. Cong, X. Guo, S.X. Chen and Y.L. Fang
The cucumber (Cucumis sativus L.) is an important crop grown worldwide. In this study, the genetic diversity of 42 cucumber cultivars in China was analyzed using 51 pairs of simple sequence repeat (SSR) primers. These primers identified 129 polymorphic loci, 95.6% of which were polymorphic. The mean effective number of alleles, mean Nei’s gene diver.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.10
- DOI:
- 10.4238/2015.December.29.10
Circulating miR-125b as a biomarker of Ewing�s sarcoma in Chinese children
Authors: C.L. Nie, W.H. Ren, Y. Ma, J.S. Xi and B. Han
Previous studies indicated that microRNA-125b (miR-125b) has an important role in the progression of Ewing’s sarcoma (ES). The purpose of the current study was to examine expression changes of miR-125b in the serum of ES patients and evaluate if the expression level of miR-125b could serve as a new biomarker for ES. This study was performed on patie.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.12
- DOI:
- 10.4238/2015.December.29.12
Characterization and functional analysis of a chitin synthase gene (HcCS1) identified from the freshwater pearlmussel Hyriopsis cumingii
Authors: H.F. Zheng, Z.Y. Bai, J.Y. Lin, G.L. Wang, J.L. Li
The triangle sail mussel, Hyriopsis cumingii, is the most important freshwater pearl mussel in China. However, the mechanisms underlying its chitin-mediated shell and nacre formation remain largely unknown. Here, we characterized a chitin synthase (CS) gene (HcCS1) in H. cumingii, and analyzed its possible physiological function. The complete ORF sequence.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.36
- DOI:
- 10.4238/2015.December.29.36
Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5],12:i:-
Authors: M.P.O. Tomiyama, C.H. Werle, G.P. Milanez, D.B. N�³brega, J.P. Pereira, A.P. Calarga, F. Flores and M. Brocchi
Salmonella enterica subsp enterica serovar 4,5,12:i:- has been responsible for many recent Salmonella outbreaks worldwide. Several studies indicate that this serovar originated from S. enterica subsp enterica serovar Typhimurium, by the loss of the flagellar phase II gene (fljB) and adjacent sequences. However, at least two different clones of S. enterica.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.13
- DOI:
- 10.4238/2015.December.29.13
Association of SNPs in the PPAR�³ gene and hypertension in a Mongolian population
Authors: L. Yang, R.G. Tian, P.Y. Chang, M.R. Yan and X.L. Su
The association of single nucleotide polymorphisms (SNPs) in PPARγ with hypertension is controversial. The aim of the present study was to clarify the contributions of PPARγ genetic variants to hypertension through an association study. A total of 414 unrelated Mongolian herdsmen and 524 Han farmers were included in this study. Fourteen intron.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.39
- DOI:
- 10.4238/2015.December.29.39
Investigations into the association between polymorphisms in the interleukin-10 gene and risk of early-onset preeclampsia
Authors: Q.Y. Liu, F.Y. Gao, X.R. Liu, J. Li, M. Ji, J. Dong and X.T. Wang
In this case-control study, we assessed the influence of IL-10 -1082A/G and -819T/C on the development of preeclampsia. The IL-10 -1082A/G and -819T/C polymorphisms were assessed by polymerase chain reaction-restriction fragment length polymorphism. The genotype distributions of the IL-10 -1082A/G and -819T/C polymorphisms in the control subjects were in .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.42
- DOI:
- 10.4238/2015.December.29.42
Two novel ATP2C1 mutations in patients with Hailey-Hailey disease and a literature review of sequence variants reported in the Chinese population
Authors: L. Meng, Y. Gu, X.F. Du, M.H. Shao, L.L. Zhang, G.L. Zhang and X.L. Wang
Hailey-Hailey disease (HHD) is an autosomal dominant disorder in which the ATP2C1 gene has been implicated. Many mutations of this gene have been detected in HHD patients. To analyze such mutations in HHD and summarize all those identified in Chinese patients with this disease, we examined four familial and two sporadic cases and searched for case reports.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.45
- DOI:
- 10.4238/2015.December.29.45
Linker length affects expression and bioactivity of the onconase fusion protein in Pichia pastoris
Authors: G.G. Yang, X.Y. Xu, Y. Ding, Q.Q. Cui, Z. Wang, Q.Y. Zhang, S.H. Shi, Z.Y. Lv, X.Y. Wang, J.H. Zhang, R.G. Zhang and C.S. Xu
The aim of this study was to analyze the effect of linker length on the expression and biological activity of recombinant protein onconase (ONC) in fusion with human serum albumin (HSA) in Pichia pastoris. Four flexible linkers with different lengths namely Linker L0, L1: (GGGGS)1, L2: (GGGGS)2, and L3:(GGGGS)3 were inserted into the fusion gene and refer.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.46
- DOI:
- 10.4238/2015.December.29.46