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SNPs in the 5'-regulatory region of the tyrosinase gene do not affect plumage color in ducks (Anas platyrhynchos)
Authors: N.N. Zhang, J.W. Hu, H.H. Liu, H.Y. Xu, H. He and L. Li
Tyrosinase, encoded by the TYR gene, is the rate-limiting enzyme in the production of melanin pigment. In this study, plumage color separation was observed in Cherry Valley duck line D and F1 and F2 hybrid generations of Liancheng white ducks. Gene sequencing and bioinformatic analysis were applied to the 5'-regulatory region of TYR, to explore the connec.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.11
- DOI:
- 10.4238/2015.December.28.11
Construction and characterization of recombinant adenovirus carrying a mouse TIGIT-GFP gene
Authors: J.M. Zheng, J.L. Cui, W.T. He, D.W. Yu, Y. Gao, L. Wang, Z.K. Chen and H.M. Zhou
Recombinant adenovirus vector systems have been used extensively in protein research and gene therapy. However, the construction and characterization of recombinant adenovirus is a tedious and time-consuming process. TIGIT is a recently discovered immunosuppressive molecule that plays an important role in maintaining immunological balance. The constructio.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.14
- DOI:
- 10.4238/2015.December.28.14
Expression profiles of differentially expressed genes affecting fecundity in goat ovarian tissues
Authors: Y.H. Ling, Q. Quan, H. Xiang, L. Zhu, M.X. Chu, X.R. Zhang and C.Y. Han
Although RNA-Seq is an effective method for identifying and exploring novel functional genes in mammals, it has rarely been applied to study fertility-related genes in the goat. In this study, RNA-Seq was used to screen the estrus ovaries of uniparous and multiparous Anhui white goats (AWGs). In total, 15,890 genes were identified and 2201 of these were f.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.23
- DOI:
- 10.4238/2015.December.28.23
Downregulation of serum DKK-1 predicts poor prognosis in patients with papillary thyroid cancer
Authors: Y.P. Zhao, W. Wang, X.H. Wang, Y. Xu, Y. Wang, Z.F. Dong and J.J. Zhang
The Wnt inhibitor dickkopf-1 (DKK-1) has been shown to be closely correlated with tumor initiation and progression in various types of cancers. However, the serum level of DKK-1 in patients with papillary thyroid cancer (PTC) and its potential clinical significance is poorly understood. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the le.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.38
- DOI:
- 10.4238/2015.December.28.38
Analysis of specific sequences in mutant rice generated by introduction of exogenous corn DNA
Authors: W.C. Li, S.D. Ji, C.Z. Tian, Z.K. Li, X.C. Wang, H.C. Zhang, Y.L. Sun, X.Z. Shi, T.X. Wang and J.Y. Li
Rice variation induced by the introduction of exogenous DNA has become an important method of improving rice varieties and creating new germplasms. In this study, we transferred maize genomic DNA fragments to the receptor of Nipponbare rice using a modified “pollen-tube pathway” method. Material from mutant rice B1 and B2 were acquired and 14 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.5
- DOI:
- 10.4238/2015.December.29.5
Transplantation of erythropoietin gene-transfected umbilical cord mesenchymal stem cells as a treatment for limb ischemia in rats
Authors: J.P. Li, D.W. Wang and Q.H. Song
The aim of this study was to investigate the mechanisms of erythropoietin (EPO)-transfected umbilical cord mesenchymal stem cells (UC-MSCs) in the treatment of rats with ischemic limb and provide a theoretical basis for optimization of UC-MSC transplantation. Sixty SD rats were randomly divided into four groups: ischemia control group, EPO treatment group.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.8
- DOI:
- 10.4238/2015.December.29.8
Isolation, characterization, and multiplexing of novel microsatellite markers for the tropical scalloped spiny lobster (Panulirus homarus)
Authors: M. Delghandi, S. Goddard, D.R. Jerry, H.T. Dao, H. Afzal and S.S. Al-Jardani
Of the various spiny lobster species in the tropical and subtropical Indo-West Pacific region, the tropical scalloped spiny lobster (Panulirus homarus) supports one of the most commercially valuable fishery resources in many coastal African and Asian countries. The last decade has witnessed a serious decline in the wild populations of this species. Knowle.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.14
- DOI:
- 10.4238/2015.December.29.14
Glucagon-like peptide-1 receptor agonists inhibit hepatic stellate cell activation by blocking the p38 MAPK signaling pathway
Authors: L.K. Wu, Y.C. Liu, L.L. Shi and K.D. Lu
We investigated the effects of glucagon-like peptide-1 receptor (GLP-1R) agonists on p38 mitogen-activated protein kinase (MAPK) signaling during inhibition of hepatic stellate cell (HSC) activity. Human HSCs were cultured and morphologically identified. HSC samples were collected and randomly divided into three groups (N = 20 samples per group): a contro.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.17
- DOI:
- 10.4238/2015.December.29.17
Association between MTHFR gene polymorphisms (C677T, A1298C) and genetic susceptibility to prostate cancer: a meta-analysis
Authors: P.L. Chen, W.T. Li, J. Wang, Y.D. Jiang, P. Wu, T. Chen and S.B. Zheng
Genetic polymorphisms (C677T and A1298C) in methylenetetrahydrofolate reductase (MTHFR) were shown to be related to prostate cancer risk in previous studies; however, the results are controversial. We performed a meta-analysis of previous studies and quantitatively estimated these associations. Pubmed, Embase, and Cochrane Library Database were searched f.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.29
- DOI:
- 10.4238/2015.December.29.29
Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery
Authors: L.Y. Yu, J.J. Bai, J.J. Fan, D.M. Ma, Y.C. Quan and P. Jiang
Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.35
- DOI:
- 10.4238/2015.December.29.35