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Evaluation of the mutagenic activity of hydroxyurea on the G1-S-G2 phases of the cell cycle: an in vitro study
Authors: PatrÃ?Âcia Danielle Lima de Lima, PlÃ?Ânio Cerqueira dos Santos Cardoso, AndrÃ?© Salim Khayat, Marcelo de Oliveira Bahia, Rommel RodrÃ?Âguez Burbano
Hydroxyurea is considered an antineoplastic drug, which also plays an important role in the treatment of sickle cell anemia patients. We evaluated and compared the clastogenic and cytotoxic effects of hydroxyurea, using chromosomal aberrations and mitotic index, respectively, as endpoints. In vitro short-term cultures of lymph.. Read More»
- Genet. Mol. Res. 2(3):
Is the number of antennal plate organs (sensilla placodea) greater in hygienic than in non-hygienic Africanized honey bees?
Authors: K�¡tia Peres Gramacho, Lionel Segui Gon�§alves, Ant�´nio Carlos Stort, Adriana Backx Noronha
Hygienic behavior is a desirable trait in honey bees (Apis mellifera L.), as hygienic bees quickly remove diseased brood, interrupting the infectious cycle. Hygienic lines of honey bees appear to be more sensitive to the odors of dead and diseased honey bee brood, and Africanized honey bees are generally more hygienic than are.. Read More»
- Genet. Mol. Res. 2(3):
Genetic, biochemical, and anatomic characterization of neurological mutant 3, a new mouse model for Parkinson�s disease
Authors: Marie E. Legare, William H. Hanneman
We have identified a new mutant mouse that we have named new mouse neurological mutant 3 (NM3); it may be a useful model to understand the underlying molecular and genetic basis of Parkinson’s disease (PD). A mouse carrying the NM3 mutation arose spontaneously in an RIIIS/J breeding colony and was identified as having a .. Read More»
- Genet. Mol. Res. 2(3):
Non-Darwinian and Darwinian prokaryotic and eukaryotic evolution - an enigma in cell biology conservation
Authors: Hugo Hoenigsberg
Our theory is embarrassingly simple. What made today’s prokaryotes and modern cyanobacteria so robust is the fact that in their origin, back in the Archean (3 billion years ago), selection did not play a central role in evolution, it had only a transitory role. Asexual reproduction, mutation, drift and sampling variance .. Read More»
- Genet. Mol. Res. 2(3):
Mining microorganism EST databases in the quest for new proteins
Authors: Alessandra Concei�§�£o Faria-Campos, Gustavo Coutinho Cerqueira, Charles Anacleto, Jos�© Miguel Ortega, Cl�¡udia M�¡rcia Benedetto de Carvalho
Microorganisms with large genomes are commonly the subjects of single-round partial sequencing of cDNA, generating expressed sequence tags (ESTs). Usually there is a great distance between gene discovery by EST projects and submission of amino acid sequences to public databases. We analyzed the relationship between available E.. Read More»
- Genet. Mol. Res. 2(1):
Sex in fungi: lessons of gene regulation
Authors: Cristina A.J. Souza, Carla C. Silva, Adlane V.-B. Ferreira
Fungi have been very useful for gene regulation studies. Mating implicates in a series of events influenced by many types of environmental input that are interpreted into regulatory pathways, including signal transduction. Although various aspects of mating and signal transduction in the yeast Saccharomyces cerevisiae have lon.. Read More»
- Genet. Mol. Res. 2(1):
Evaluation of alternative reporter genes for the yeast two-hybrid system
Authors: Alessandra L. Starling, Kenneth J. Gollob, Elisabete J. Vicente, Gisele M. Andrade-N�³brega, M�´nica B. Rodriguez, J. Miguel Ortega
The yeast two-hybrid system is a powerful tool for screening protein-protein interactions and has also been used for large-scale studies. We evaluated two protein-coding sequences as reporter genes for the yeast two-hybrid system, to determine if it was suitable as an alternative screening strategy. Aspergillus awamori glucoam.. Read More»
- Genet. Mol. Res. 2(1):
Cell biology, molecular embryology, Lamarckian and Darwinian selection as evolvability
Authors: H. Hoenigsberg
The evolvability of vertebrate systems involves various mechanisms that eventually generate cooperative and nonlethal functional variation on which Darwinian selection can operate. It is a truism that to get vertebrate animals to develop a coherent machine they first had to inherit the right multicellular ontogeny. The ontogen.. Read More»
- Genet. Mol. Res. 2(1):
New genomic resources for the honey bee(Apis mellifera L.): development of a deep-coverage BAC library and a preliminary STC database
Authors: J.P. Tomkins, M. Luo, G.C. Fang1, D. Main1, J.L. Goicoechea,M. Atkins, D.A. Frisch, R.E. Page, E. Guzm�¡n-Novoa5, Y. Yu,G. Hunt6 and R.A. Wing
We have constructed a bacterial artificial chromosome (BAC) library for a European honey bee strain using the cloning enzyme HindIII in order to develop resources for structural genomics research. The library contains 36,864 clones (ninety-six 384-well plates). A random sampling of 247 clones indicated an average insert size o.. Read More»
- Genet. Mol. Res. 1(4):
(Co)variance components, genetic parameters and annual trends for calf weights in a Brahman herd kept on floodable savanna
Authors: Dieter Plasse1, Omar Verde, Jes�ºs Arango, Luis Camaripano, Hugo Fossi, Rafael Romero, Carlos Rodriguez M. and Jos�© L. Rumbos
(Co)variance components and genetic parameters were estimated for body weights of an elite Brahman herd under a designed, supervised management and genetic program, including strategic artificial insemination (AI). Restricted maximum likelihood methods were used with a univariate animal model for birth weight (BW) and a bivari.. Read More»
- Genet. Mol. Res. 1(4):