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Simple Sequence Repeats

Plant Genetics   Research Article

Genetic diversity and population structure of ancient Platycladus orientalis (Cupressaceae) in the Mausoleum of the Yellow Emperor revealed by SSR

Authors: Yao Xu1, Jiantao Zhao, Sheng Zhang, Zhong Zhao

Ancient trees can serve as genetic reservoirs for special genes, and they are of significance in genetic applications dealing with forest fragmentation, climate change and drought. The Mausoleum of the Yellow Emperor in Shaanxi China has the largest number of ancient Platycladus orientalis trees. Here, Simple sequence repeat (SSR) technique was used to st.. Read More»

Genet. Mol. Res. 17(4):
http://dx.doi.org/gmr16039924
DOI:
http://dx.doi.org/10.4238/gmr16039924
Human Genetics   Research Article

Development and characterization of novel transcriptome-derived microsatellites for genetic analysis of persimmon

Authors: C. Luo, Q.L. Zhang and Z.R. Luo

Oriental persimmon (Diospyros kaki Thunb.) (2n = 6x = 90) is a major commercial and deciduous fruit tree that is believed to have originated in China. However, rare transcriptomic and genomic information on persimmon is available. Using Roche 454 sequencing technology, the transcriptome from RNA of the flowers of D. kaki was a.. Read More»

Genet. Mol. Res. 13(2):
2014.April.16.10
DOI:
10.4238/2014.April.16.10
Plant Genetics   Research Article

Identification of orchardgrass (Dactylis glomerata L.) cultivars by using simple sequence repeat markers

Authors: L.-F. Jiang1, X.-Q. Zhang1, X. Ma1, L.-K. Huang1, W.-G. Xie2, Y.-M. Ma1 and Y.-F. Zhao1

The accurate identification of orchardgrass (Dactylis glomerata L.) cultivars is necessary to ensure purity for consumers, the effective utilization of cultivars, and to protect the intellectual property for breeders. Therefore, this study aimed to use SSR to construct DNA fingerprinting of orchardgrass cultivars. The genetic diversity of 32 orchardgrass .. Read More»

Genet. Mol. Res. 12(4):
Animal Genetics   Research Article

Genetic diversity and differentiation in natural populations of Arapaima gigas from lower Amazon revealed by microsatellites

Authors: P.F. Fazzi-Gomes, N. Melo, G. Palheta, S. Guerreiro, M. Amador, A.K. Ribeiro-dos-Santos, S. Santos and I. Hamoy

Genetic variability is one of the important criteria for species conservation decisions. This study aimed to analyze the genetic diversity and the population differentiation of two natural populations of Arapaima gigas, a species with a long history of being commercially exploited. We collected 87 samples of A. gigas from Gran.. Read More»

Genet. Mol. Res. 16(1):
gmr16019552
DOI:
10.4238/gmr16019552
Plant Genetics   Research Article

Phenotypic and molecular characterization of a tomato (Solanum lycopersicum L.) F2 population segregation for improving shelf life

Authors: K.N. Yogendra P.H. Ramanjini Gowda

Breeding for better quality fruits is a major focus for tomatoes, which are continuously subjected to post-harvest losses. Several methods have been used to improve the fruit shelf life of tomatoes, including the use of ripening gene mutants of Solanum lycopersicum. We developed extended shelf-life tomato hybrids with better quality fruits using ripening .. Read More»

Genet. Mol. Res. 12(1):
2013.January.9.4
DOI:
10.4238/2013.January.9.4
Human Genetics   Research Article

Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.)

Authors: Z.J. Yang, Z.S. Peng and H. Yang

Simple sequence repeats (SSRs) are highly informative, polymorphic, and co-dominant Mendelian markers that provide an important genomic resource for genetic research. Recently, the use of large-scale transcriptome sequence has become a reliable and efficient approach for the identification and development of new EST-SSR markers. In this study, 8389 potent.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017509
DOI:
10.4238/gmr.15017509
Plant Genetics   Research Article

Marker-trait association analysis for agronomic and compositional traits in sainfoin (Onobrychis viciifolia)

Authors: K. Kempf, C.S. Malisch, C. Grieder, F. Widmer and R. K�¶lliker

Sainfoin (Onobrychis viciifolia) is a perennial forage legume with great potential for use in sustainable agriculture due to its low input requirements, good drought tolerance, and production of forage rich in polyphenolic compounds, which are beneficial for animal health. However, its distribution and cultivation are limited .. Read More»

Genet. Mol. Res. 16(1):
gmr16019483
DOI:
10.4238/gmr16019483
Plant Genetics   Research Article

Development, characterization, and annotation of potential simple sequence repeats by transcriptome sequencing in pears (Pyrus pyrifolia Nakai)

Authors: H. Zhou, B.H. Cai, Z.Q. L�¼, Z.H. Gao and Y.S. Qiao

Simple sequence repeats (SSRs), one of the most powerful molecular markers, can be used for DNA fingerprinting, variety identification, genetic mapping, and marker-assisted selection. Using the pear’s (Pyrus pyrifolia Nakai) 75,764 unigenes (55,676,271 bp) obtained by deep transcriptome sequencing, a total of 10,622 novel SSRs were identified in 915.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038683
DOI:
10.4238/gmr.15038683
Plant Genetics   Research Article

Comparison of a retrotransposon-based marker with microsatellite markers for discriminating accessions of Vitis vinifera

Authors: G.C. Santâ��Ana, J.L. Ferreira, H.S. Rocha, A. Bor�©m, M. Pasqual and G.M.A. Can�§ado

Identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. Recently, new types of molecular markers have been developed, such as retrotransposon-based markers. Because of their multilocus pattern, retrotransposon-based markers might be able to differentiat.. Read More»

Genet. Mol. Res. 11(2):
2012.May.21.8
DOI:
10.4238/2012.May.21.8
Plant Genetics   Research Article

Characterization of nine transferred SSR markers in the tropical tree species Enterolobium contortisiliquum (Fabaceae)

Authors: P.A. Moreira S.A.S. Sousa F.A. Oliveira N.H. Ara�ºjo G.W. Fernandes D.A. Oliveira

Transfer of molecular markers is widely used in conservation genetic studies. We investigated the transferability of simple sequence repeat (SSR) markers developed for Enterolobium cyclocarpum to E. contortisiliquum, a tropical tree widely distributed in dry forests. A set of 9 evaluated SSR markers were amplified in E. contortisiliquum and the degree of .. Read More»

Genet. Mol. Res. 11(4):
2012.August.17.11
DOI:
10.4238/2012.August.17.11