Simple Sequence Repeats
Genetic dissection of upland cotton (Gossypium hirsutum) cultivars developed in Hubei Province by mapped SSRs
Authors: J.L. Tu, M.J. Zhang, X.Q. Wang, X.L. Zhang and Z.X. Lin
The genetic diversity of 51 upland cotton cultivars with different parental origins and breeding periods that were developed in Hubei Province was studied on the basis of 237 mapped simple sequence repeat markers covering the cotton genome. A total of 108 polymorphic primer pairs amplified 196 loci; the polymorphism information content range was 0.04 to 0.83.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.31.4
- DOI:
- 10.4238/2014.January.31.4
Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix
Authors: M. Cristancho and C. Escobar
The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR m.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr493
- DOI:
- 10.4238/vol7-4gmr493
Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers
Authors: Alexandre Missiaggia and Dario Grattapaglia
We extended the concept of fluorescent microsatellite genotyping with a single-universal tailed primer to the simultaneous use of three different tailed primers to allow multiplexed 4-color detection for medium throughput genotyping of plant species. The method was tested on Eucalyptus DNA samples using three forward primer se.. Read More»
- Genet. Mol. Res. 5(1):
Genetic structure of the threatened Dipterocarpus costatus populations in lowland tropical rainforests of southern Vietnam
Authors: N.M. Duc, V.D. Duy, B.T.T. Xuan, B.V. Thang, N.T.H. Ha and N.M. Tam
Dipterocarpus costatus is an endangered species restricted to the lowland forests of southern Vietnam. Habitat loss and over-exploitation of D. costatus wood are the major threats to this species. We investigated the level of genetic variability within and among populations of D. costatus in order to provide guidelines for the.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048821
- DOI:
- 10.4238/gmr15048821
Genetic analysis of molecular markers for propamocarb residue in Cucumis sativus using quantitative trait locus mapping
Authors: M. Xin, L. Wang, B.H. Ma, Z.W. Qin and X.Y. Zhou
The use of pesticides to protect plants against harmful organisms, such as pathogenic microorganisms, is one of the most effective ways to improve agricultural production. However, the continuous use of pesticides might present a risk to human health, animals, and the environment. In this study, two cucumber (Cucumis sativus) .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048776
- DOI:
- 10.4238/gmr15048776
Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers
Authors: P.P. Batista, J.F. Santos, N.T. Oliveira, A.P.D. Pires, C.M.S. Motta and E.A. Luna-Alves Lima
The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3gmr422
- DOI:
- 10.4238/vol7-3gmr422
Microsatellite markers derived from Japanese scallop (Mizuhopecten yessoensis) expressed sequence tags
Authors: Y.F. Li, X.G. Gao, W.D. Liu, Y. Tian, L. Gao, S.L. Li and C.B. He
Japanese scallop (Mizuhopecten yessoensis) is a cold-water shellfish, and a species of economic importance in China. In this study, we developed and evaluated simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs) of M. yessoensis. The characteristics of 12 EST-SSR loci were investigated in 30 individual scallops, and the result reve.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.3
- DOI:
- 10.4238/2014.March.24.3
Genetic variation and comparison of orchardgrass (Dactylis glomerata L.) cultivars and wild accessions as revealed by SSR markers
Authors: W.G. Xie, X.F. Lu, X.Q. Zhang, L.K. Huang and L. Cheng
Orchardgrass is a highly variable, perennial forage grass that is cultivated throughout temperate and subtropical regions of the world. Despite its economic importance, the genetic relationship and distance among and within cultivars are largely unknown but would be of great interest for breeding programs. We investigated the .. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.24.1
- DOI:
- 10.4238/2012.February.24.1
Characterization and comparison of EST-SSR and TRAP markers for genetic analysis of the Japanese persimmon Diospyros kaki
Authors: C. Luo, F. Zhang, Q.L. Zhang, D.Y. Guo and Z.R. Luo
We developed and characterized expressed sequence tags (ESTs)-simple sequence repeats (SSRs) and targeted region amplified polymorphism (TRAP) markers to examine genetic relationships in the persimmon genus Diospyros gene pool. In total, we characterized 14 EST-SSR primer pairs and 36 TRAP primer combinations, which were amplified across 20 germplasms of .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.9.3
- DOI:
- 10.4238/2013.January.9.3
Survey of simple sequence repeats in woodland strawberry (Fragaria vesca)
Authors: L. Guan, J.F. Huang, G.Q. Feng, X.W. Wang, Y. Wang, B.Y. Chen and Y.S. Qiao
The use of simple sequence repeats (SSRs), or microsatellites, as genetic markers has become popular due to their abundance and variation in length among individuals. In this study, we investigated linkage groups (LGs) in the woodland strawberry (Fragaria vesca) and demonstrated variation in the abundances, densities, and relative densities of mononucleot.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.3
- DOI:
- 10.4238/2013.July.30.3