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Ssr Marker

Microbial Genetics   Research Article

Transferability and characterization of simple sequence repeat markers from Anacardium occidentale to A. humile (Anacardiaceae)

Authors: L.G. Cota P.A. Moreira E.V. Menezes A.S. Gomes A.R.O. Ericsson D.A. Oliveira A.F. Melo Jr.

Use of molecular markers can be limited by the high cost and extensive time required for their development. Transfer of simple sequence repeat (SSR) markers reduces the cost and time limitations and has allowed the use of these markers in a larger number of species. We tested 11 SSR markers previously developed for Anacardium occidentale on A. humile. The.. Read More»

Genet. Mol. Res. 11(4):
2012.October.17.7
DOI:
10.4238/2012.October.17.7
Plant Genetics   Research Article

Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers

Authors: M.C. Guidugli, R. Ferreira-Ramos, A.C.B. de Sousa, F.W. Cidade, T.G. Marconi, M.A. Mestriner, M. Groppo and A.L. Alzate-Marin

Metrodorea nigra (Rutaceae) is an endemic Brazilian tree of great ecological importance, frequently found in the submontane regions of ombrophilous dense and semideciduous forests. This tree is useful for reforesting degraded areas and the wood can be employed in construction. We developed 12 microsatellite markers from a geno.. Read More»

Genet. Mol. Res. 11(1):
http://dx.doi.org/2012.January.9.2
DOI:
http://dx.doi.org/10.4238/2012.January.9.2
Plant Genetics   Research Article

Transcriptome analysis of potential simple sequence repeat markers in Ammopiptanthus mongolicus

Authors: M. Jin, M.Y. Guo, L. Han, J.L. Li, S.Y Yang and Y.H. Su

Ammopiptanthus mongolicus, an evergreen broadleaf legume shrub, can survive under conditions of high and low temperature, extreme salinity, and drought. This attribute makes it an ideal model for studying mechanisms of stress tolerance in plants. However, simple sequence repeat (SSR) resources for this species are insufficient.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038581
DOI:
10.4238/gmr.15038581
Medical Genetics   Research Article

Discrimination of common bean cultivars using multiplexed microsatellite markers

Authors: P.C.B. Cardoso, C. Brondani, I.P.P. Menezes, P.A.M.R. Valdisser, T.C.O. Borba,, M.J. Del Peloso and R.P. Vianello

Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite .. Read More»

Genet. Mol. Res. 13(1):
2014.March.24.1
DOI:
10.4238/2014.March.24.1
Animal Genetics   Research Article

Effect of fragmentation on the natural genetic diversity of Theobroma speciosum Willd. ex Spreng. populations

Authors: T.L. Varella, A.A.B. Rossi, J.F.E. Dardengo, G.F. Silveira, M.D.A. Souza and M.L.S. Carvalho

t fragmentation reduces the effective size of natural populations, isolates individuals in the landscape, and, consequently, changes species’ mating systems by increasing the degree of relatedness between individuals and inbreeding. Investigating the impact of habitat degradation on forest fragments helps to assess the g.. Read More»

Genet. Mol. Res. 15(4):
gmr15048630
DOI:
10.4238/gmr15048630
Plant Genetics   Research Article

Evaluation of wheat landrace genotypes for salinity tolerance at vegetative stage by using morphological and molecular markers

Authors: A. Shahzad, M. Ahmad, M. Iqbal, I. Ahmed and G.M. Ali

Identification of new sources of salt tolerance is particularly important to develop crop varieties suitable for saline soils. We evaluated 129 Pakistani and 58 exotic wheat landraces/cultivars grown in Hoagland’s hydroponic nutrient solution, under control (tap water equivalent to 10 mM salt) and salt stress (200 mM NaC.. Read More»

Genet. Mol. Res. 11(1):
2012.March.19.2
DOI:
10.4238/2012.March.19.2
Animal Genetics   Research Article

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Authors: Z.-W. Chen, R.-J. Lu, L. Zou, Z.-Z. Du, R.-H. Gao, T. He and J.-H. Huang

We analyzed the genetic diversity of 115 barley germplasms, including 112 landraces and three new barley cultivars grown in the Shanghai region, using a set of 11 SSR markers. Sixty-six alleles were observed at the 11 SSR loci, ranged from three to ten, with a mean of six alleles per locus. The polymorphism information content ranged from 0.568 to 0.853, .. Read More»

Genet. Mol. Res. 11(1):
2012.March.16.2
DOI:
10.4238/2012.March.16.2
Plant Genetics   Research Article

Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers

Authors: M.Q.U. Farooqi, K.J. Sa, T.K. Hong and J.K. Lee

In this study, eight inbred maize lines with high or low tolerance to cold temperature were investigated using bulk segregant analysis (BSA). Genetic diversity and genetic relationships were investigated using 100 simple sequence repeat (SSR) markers linked to cold tolerance in maize. A total of 319 alleles were identified. Am.. Read More»

Genet. Mol. Res. 15(4):
gmr15049326
DOI:
10.4238/gmr15049326
Human Genetics   Research Article

Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers

Authors: G.Q. Wei, L. Yu, C.L. Liu, B.J. Zhu and H.J. Ding

In the silkworm, Bombyx mori, normal markings are mainly controlled by the +P gene, which is located on the second chromosome. Due to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the +P gene based on an SSR linkage map using silkworm strains P50 and H9, which are normal marking.. Read More»

Genet. Mol. Res. 12(3):
2013.January.22.1
DOI:
10.4238/2013.January.22.1
Plant Genetics   Research Article

Population structure of Annona crassiflora: an endemic plant species of the Brazilian Cerrado

Authors: R. Gwinner, T.A. Setotaw, F.A. Rodrigues, D.V.C. Fran�§a, F.A. da Silveira, L.A.S. Pio and M. Pasqual

Habitat fragmentation has numerous consequences, particularly to endemic species, and has a negative impact on the genetic diversity of neglected species, leading to genetic drift. Annona crassiflora Mart. is a species that is endemic to Brazil, and its incidence in the Cerrado biome has decreased. The identification and chara.. Read More»

Genet. Mol. Res. 15(4):
gmr15049137
DOI:
10.4238/gmr15049137