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Ssr

Bioinformatics   Research Article

Taxonomic status of Pinus henryi using multiplexed microsatellite markers

Authors: Q. Xie, Z.-H. Liu and Z.-Q. Li

The taxonomic status of Pinus henryi, a rare species endemic to China, is still ambiguous. In this study, the genetic relationships among P. henryi and its congeners (P. tabulaeformis, P. tabulaeformis var. mukdensis, and P. massoniana) were revealed using multiplexed microsatellite markers, including chloroplast microsatellites, nuclear microsatellites, .. Read More»

Genet. Mol. Res. 14(2):
2015.May.4.13
DOI:
10.4238/2015.May.4.13
Animal Genetics   Research Article

Molecular genetic analysis of heterosis in interspecific hybrids of Argopecten purpuratus x A. irradians irradians

Authors: L.P. Hu, X.T. Huang, Y. Sun, J.X. Mao, S. Wang, C.D. Wang and Z.M. Bao

Argopecten purpuratus and Argopecten irradians irradians hybridization was successfully performed and the hybrid offspring displayed apparent heterosis in growth traits. To better understand the genetic basis of heterosis, the genomic composition and genetic variation of the hybrids were analyzed with amplified fragment length polymorphism (AFLP) and simp.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.9.9
DOI:
http://dx.doi.org/10.4238/2015.September.9.9
Human Genetics   Research Article

De novo assembly and characterization of Gleditsia sinensis transcriptome and subsequent gene identification and SSR mining

Authors: S. Han, Z. Wu, X. Wang, K. Huang, Y. Jin, W. Yang and H. Shi

Gleditsia sinensis is a Chinese native deciduous tree with a high economic and medicinal value. However, there is limited knowledge on the molecular processes responsible for the medical properties of this species owing to lack of bioinformatic resources such as available whole-genome sequences. In the present study, RNA sequencing data were used to analy.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017740
DOI:
10.4238/gmr.15017740
Plant Genetics   Research Article

Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress

Authors: M.R. Naroui Rad M. Abdul Kadir M.Y. Rafii H.Z.E. Jaafar M.R. Naghavi

This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under dr.. Read More»

Genet. Mol. Res. 11(4):
2012.November.12.5
DOI:
10.4238/2012.November.12.5
Plant Genetics   Research Article

Assessing molecular and morpho-agronomical diversity and identification of ISSR markers associated with fruit traits in quince (Cydonia oblonga)

Authors: I. Ganopoulos, G. Merkouropoulos, S. Pantazis, C. Tsipouridis and A. Tsaftaris

Quince is a deciduous tree known to the countries around the Mediterranean since antiquity. Nowadays, quince is used as an ornamental plant, and as a rootstock for pear trees, with its fruit being appreciated mainly for production of jam and sweets rather than for raw consumption. Quince leaves contain compounds with antioxida.. Read More»

Genet. Mol. Res. 10(4):
2011.November.4.7
DOI:
10.4238/2011.November.4.7
Plant Genetics   Research Article

Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq

Authors: X.F. Zheng, Y.N. You, Y. Diao, X.W. Zheng, K.Q. Xie, M.Q. Zhou, Z.L. Hu and Y.W. Wang

Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.22.11
DOI:
http://dx.doi.org/10.4238/2015.September.22.11
Plant Genetics   Research Article

Morphoagronomic and molecular profiling of Capsicum spp from southwest Mato Grosso, Brazil

Authors: A.L. Campos, T.N. Marostega, N.S.S. Cabral, K.L. Ara�ºjo, M.E. Serafim, S. Seabra-J�ºnior, C.P. Sudr�©, R. Rodrigues and L.G. Neves

The genus Capsicum ranks as the second most exported vegetable in Brazil, which is also considered to be a center of diversity for this genus. The aim of this study was to rescue genetic variability in the genus Capsicum in the southwest region of Mato Grosso, and to characterize and estimate the genetic diversity of accessions based on morphoagronomic de.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038167
DOI:
10.4238/gmr.15038167
Plant Genetics   Research Article

Development of expressed sequence tag-based microsatellite markers for the critically endangered Iso�«tes sinensis (Isoetaceae) based on transcriptome analysis

Authors: A.W. Gichira, Z.C. Long, Q.F. Wang, J.M. Chen and K. Liao

Isoëtes sinensis is a critically endangered quillwort. To facilitate studies on the conservation genetics of this species, we developed expressed sequence tag-simple sequence repeat (EST-SSR) markers. A total of 50,063 unigenes were predicted by transcriptome sequencing, 5294 (10.6%) of which significantly matched 3011 Gene Ontology annotations and 2.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038497
DOI:
10.4238/gmr.15038497
Cytogenomics   Research Article

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species

Authors: G.M. Guimar�£es-Marques J.S. Batista H.M. Guimar�£es M.V. Naice-Daou M.P. Lima K.M. Formiga J.M.M. Santos C.A.P. Lima M.S. Rafael

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity rang.. Read More»

Genet. Mol. Res. 11(4):
2012.December.6.3
DOI:
10.4238/2012.December.6.3
Plant Genetics   Research Article

Transferability and utility of white oat (Avena sativa) microsatellite markers for genetic studies in black oat (Avena strigosa)

Authors: P.R. Da-Silva, S.C.K. Milach and L.M. Tisian

Preservation and use of wild oat species germplasm are essential for further improvement of cultivated oats. We analyzed the transferability and utility of cultivated (white) oat Avena sativa (AACCDD genome) microsatellite markers for genetic studies of black oat A. strigosa (AsAs genome) genotypes. The DNA of each black oat g.. Read More»

Genet. Mol. Res. 10(4):
2011.November.29.2
DOI:
10.4238/2011.November.29.2