Ssr
Isolation and characterization of microsatellite markers for Dendranthema morifolium (Asteraceae) using next-generation sequencing
Authors: W.J. Yuan, S. Ye, L.-H. Du, S.-M. Li, X. Miao and F.-D. Shang
Dendranthema morifolium (Asteraceae) is a perennial herbaceous plant native to China. A long history of artificial crossings may have resulted in complex genetic background and decreased genetic diversity. To protect the genetic diversity of D. morifolium and enabling breeding of new D. morifolium cultivars, we developed a set.. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048765
- DOI:
- 10.4238/gmr.15048765
Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango
Authors: C. Luo, H.X. Wu, Q.S. Yao, S.B. Wang and W.T. Xu
Mango is one of the most commercially important fruit crops in tropical and subtropical regions. To increase the efficiency of breeding strategies, two EST-derived marker systems were developed in the present study using information from the mango fruit transcriptome. Using simple sequence repeats, 218 of 230 primer pairs showed stable amplification for 7.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.17
- DOI:
- 10.4238/2015.July.14.17
Molecular identification of Greek olive (Olea europaea) cultivars based on microsatellite loci
Authors: K. Roubos, M. Moustakas and F.A. Aravanopoulos
Olea europaea is one of the oldest species of domesticated trees. We used microsatellite markers for fingerprinting and for evaluation of genetic similarity and structure of 26 Greek olive cultivars, which cover most of the olive cultivation regions of Greece, including previously undescribed denominations from northern Greece.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr916
- DOI:
- 10.4238/vol9-3gmr916
Characterization of maize genotypes for genetic diversity on the basis of inter simple sequence repeats
Authors: R.W. Muhammad, A. Qayyum, M.Q. Ahmad, A. Hamza, M. Yousaf, B. Ahmad, M. Younas, W. Malik, S. Liaqat, E. Noor
Genetic diversity in crops is essential to make improvements related to superior germplasms. Implementation of molecular markers to identify suitable genotypes speeds up the breeding progress by enhancing selection efficiency. This study was carried out to probe genetic diversity among 21 maize genotypes using 20 inter simple .. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019438
- DOI:
- 10.4238/gmr16019438
pelB gene in isolates of Colletotrichum gloeosporioides from several hosts
Authors: L.V. Medeiros, D.B. Maciel, V.V. Medeiros, L.M. Houllou Kido and N.T. Oliveira
Colletotrichum gloeosporioides is an important pathogen for a great number of economically important crops. During the necrotrophic phase of infection by Colletotrichum spp, the degradative enzymes of plant cell walls, such as pectate lyase, clearly increase. A gene pelB that expresses a pectate lyase was identified in isolate.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr725
- DOI:
- 10.4238/vol9-2gmr725
Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays)
Authors: E.C. Oliveira, A.T. Amaral J?ºnior, L.S.A. Gon?§alves, G.F. Pena, S.P. Freitas J?ºnior, R.M. Ribeiro and M.G. Pereira
We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize h.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr767
- DOI:
- 10.4238/vol9-2gmr767
Rapid determination of transgene copy number in tobacco by competitive PCR using a pair of SSR primers
Authors: K.Y. Xu, S.H. Wang, L. Xi, Q.J. Wang, C. Dong, J.Y. Zhang, S.C. Qu and Z. Zhang
We developed a straightforward, rapid, and inexpensive method to determine transgene copy number in tobacco. The plasmid (pSSRCopy) used for tobacco transformation contains a simple sequence repeat (SSR) locus, PT1199, which was partially deleted in the middle, a homogenous SSR locus in tobacco K326. A 168-bp segment of the cl.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr790
- DOI:
- 10.4238/vol9-2gmr790
Genetic studies of â??noble caneâ? for identification and exploitation of genetic markers
Authors: S. Nawaz, F.A. Khan, S. Tabasum, M.Z. Iqbal and A. Saeed
Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei’s genetic distances for SSR data were determined and relationships between accessions were portrayed graphically i.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr795
- DOI:
- 10.4238/vol9-2gmr795
Development of EST-SSR markers in the relict tree Davidia involucrata (Davidiaceae) using transcriptome sequencing
Authors: Z.C. Long, A.W. Gichira, J.M. Chen, Q.F. Wang and K. Liao
Davidia involucrata, reputed to be a “living fossil” in the plant kingdom, is a relict tree endemic to China. Extant natural populations are diminishing due to anthropogenic disturbance. In order to understand its ability to survive in a range of climatic conditions and to design conservation strategies for this en.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048539
- DOI:
- 10.4238/gmr15048539
Population structure analysis and association mapping of blast resistance in indica rice (Oryza sativa L.) landraces
Authors: Y.Y. Wu, J.B. He, A.H. Li, N.Y. Fang, W.W. He, L.L. Dang, G.Y. Zeng, J. Huang, Y.M. Bao and H.S. Zhang
Rice blast caused by Magnaporthe oryzae is one of the most devastating rice diseases worldwide. To understand the genetic diversity of indica landrace accessions and identify simple sequence repeat (SSR) markers that are associated with blast resistance, a population of 276 indica landraces from across the world was constructed. This population was then u.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038254
- DOI:
- 10.4238/gmr.15038254