Ssr
Assessing genetic diversity of cotton cultivars using genomic and newly developed expressed sequence tag-derived microsatellite markers
Authors: Y. Zhang, X.F. Wang, Z.K. Li, G.Y. Zhang and Z.Y. Ma
Estimations of genetic diversity and of relationships between varieties are crucial for cotton breeding. The genetic diversity of 59 core cotton cultivars, most of which were collected from China’s main cotton-growing areas, was analyzed based on genomic and newly developed expressed sequence tag-derived microsatellite m.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1277
- DOI:
- 10.4238/vol10-3gmr1277
Assessment of genetic relationship among Rhododendron cultivars using amplified fragment length polymorphism and inter-simple sequence repeat markers
Authors: J.J. Xu, B. Zhao, H.F. Shen, W.M. Huang and L.X. Yuan
Genetic relationships of 17 Rhododendron cultivars, China, were assessed using inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) markers. A total of 133 bands were obtained using nine selected ISSR primers, 129 (96.99%) of which were polymorphic; 267 bands were amplified by four AFLP primer pairs, 251 (94.01%) of which .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038467
- DOI:
- 10.4238/gmr.15038467
Dephosphorylation of NSSR1 regulates alternative splicing of the GluR-B minigene
Authors: W. Zhang, L.L. Li, X.H. Chen and Z.Y. Peng
Neural salient serine/arginine-rich protein 1 (NSSR1, alternatively SRp38) is an important splicing factor that can repress pre-mRNA alternative splicing in cells during heat shock and mitosis. We show here that NSSR1 protein is dephosphorylated when cells are heat shocked or incubated with kinase inhibitor K252a. Both heat sh.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.3
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.3
Genetic diversity in natural populations of mangaba in Sergipe, the largest producer State in Brazil
Authors: A.N.R. Soares, M.F. Vit�³ria, A.L.S. Nascimento, A.S. Ledo, A.R.C. Rabbani and A.V.C. Silva
Mangaba (Hancornia speciosa Gomes) is found in areas of coastal tablelands in the Brazilian Northeast and Cerrado regions. This species has been subjected to habitat fragmentation that is mainly due to human activity, and requires conservation strategies. The aim of this study was to analyze the structure and inter- and intrapopulation genetic diversity o.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038624
- DOI:
- 10.4238/gmr.15038624
Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers
Authors: P.V. Tiago, M.P. Carneiro-Le�£o, M.L.A. Lima, N.T. Oliveira and E.A. Luna-Alves Lima
Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará d.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1069
- DOI:
- 10.4238/vol10-3gmr1069
Problems with and a system to eliminate single-primer PCR product contamination in simple sequence repeat molecular marker-assisted selection in soybean
Authors: J. Ma, S.C. Guan, D. Yao, Y.F. Wei and P.W. Wang
Polymerase chain reaction (PCR) provides a foundation for simple sequence repeat molecular marker-assisted selection (SSR MAS) in soybean. This PCR system and its various conditions have been optimized by many researchers. However, current research on the optimization of the PCR system focuses on double-primer PCR products. We.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1366
- DOI:
- 10.4238/vol10-3gmr1366
Genetic linkage map of EST-SSR and SRAP markers in the endangered Chinese endemic herb Dendrobium (Orchidaceae)
Authors: J.J. Lu S. Wang H.Y. Zhao J.J. Liu H.Z. Wang
Dendrobium officinale is an endangered orchid from southeast Asia that is known for its medicinal properties in traditional Chinese medicine. We constructed an integrated genetic linkage map of an F1 population derived from an interspecific cross between D. officinale and D. aduncum (both, 2n = 38), using expressed sequence tag-simple sequence repeats (ES.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.December.21.1
- DOI:
- 10.4238/2012.December.21.1
Transferability and characterization of simple sequence repeat markers from Anacardium occidentale to A. humile (Anacardiaceae)
Authors: L.G. Cota P.A. Moreira E.V. Menezes A.S. Gomes A.R.O. Ericsson D.A. Oliveira A.F. Melo Jr.
Use of molecular markers can be limited by the high cost and extensive time required for their development. Transfer of simple sequence repeat (SSR) markers reduces the cost and time limitations and has allowed the use of these markers in a larger number of species. We tested 11 SSR markers previously developed for Anacardium occidentale on A. humile. The.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.17.7
- DOI:
- 10.4238/2012.October.17.7
Analysis of genetic diversity among wild bermudagrass germplasm from southwest China using SSR markers
Authors: Y. Ling X.-Q. Zhang X. Ma S.-Y. Chen T.-T. Chen W. Liu
Fifty-five wild accessions of bermudagrass (Cynodon dactylon) were collected from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet), and their genetic diversity was analyzed using simple sequence repeat markers. A total of 267 polymorphic bands were detected with 18 primer combinations. The genetic similarity among the accessions ranged from 0.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.17.5
- DOI:
- 10.4238/2012.October.17.5
A protocol for high-quality genomic DNA extraction from legumes
Authors: I.O. Agbagwa S. Datta P.G. Patil P. Singh N. Nadarajan
Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding li.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.14.1
- DOI:
- 10.4238/2012.September.14.1