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Ssr

Bioinformatics   Research Article

Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)

Authors: M.P.C. Telles, F.P. Peixoto, J.S. Lima, L.V. Resende, R.P. Vianello, M.E.M.T. Walter and R.G. Collevatti

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer desig.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1026
DOI:
10.4238/vol10-1gmr1026
Human Genetics   Research Article

Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing

Authors: A.W. Gichira, Z.C. Long, G.W. Hu, R.W. Gituru, Q.F. Wang and J.M. Chen

Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individu.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028168
DOI:
10.4238/gmr.15028168
Plant Genetics   Research Article

Genetic diversity of turmeric germplasm (Curcuma longa; Zingiberaceae) identified by microsatellite markers

Authors: M.S. Sigrist, J.B. Pinheiro, J.A. Azevedo Filho and M.I. Zucchi

Turmeric (Curcuma longa) is a triploid, vegetatively propagated crop introduced early during the colonization of Brazil. Turmeric rhizomes are ground into a powder used as a natural dye in the food industry, although recent research suggests a greater potential for the development of drugs and cosmetics. In Brazil, little is k.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1047
DOI:
10.4238/vol10-1gmr1047
Plant Genetics   Research Article

Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil

Authors: R.R. Simplicio, A.M. Waldschmidt, M.B. Amorim, B.S. Almeida and D.G. Pereira

Maytenus truncata (Celastraceae) is a plant species widely used in the treatment of ulcers and tumors. Despite the intensive harvest of native specimens in the State of Bahia, northeastern Brazil, there is no information about the genetic variability or structure of this species. Therefore, the goal of this study was to estimate the genetic diversity and .. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.11
DOI:
10.4238/2015.December.23.11
Plant Genetics   Research Article

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

Authors: S. Ghariani, H. Elazreg, N. Chtourou-Ghorbel, M. Chakroun and N. Trifi-Farah

Tunisia is rich in diverse forage and pasture species including perennial ryegrass. In order to enhance forage production and improve agronomic performance of this local germplasm, a molecular analysis was undertaken. Random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and morpho-agronomical traits markers were used for genetic d.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.40
DOI:
10.4238/2015.December.23.40
Animal Genetics   Research Article

Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids

Authors: Y.D. Zhong, X.Y. Sun, E.Y. Liu, Y.Q. Li, Z. Gao and F.X. Yu

Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA.. Read More»

Genet. Mol. Res. 15(2):
Microbial Genetics   Research Article

Populations of Erythrina velutina Willd. at risk of extinction

Authors: M.F.V. Melo, L.O. Gon?§alves, A.R.C. Rabbani, S.V. ?lvares-Carvalho, J.B. Pinheiro, M.I. Zucchi and R. Silva-Mann

The goal of this study was to characterize the structure of two natural populations of the coral tree using RAPD and ISSR markers. The study evaluated all individuals in two different areas in the northeastern region of Brazil: the first was in the riparian area, 10 km x 100 m along the edge of the lower São Francisco River, and the second was in t.. Read More»

Genet. Mol. Res. 14(3):
2015.August.28.15
DOI:
10.4238/2015.August.28.15
Bioinformatics   Research Article

Isolation and characterization of polymorphic EST-SSR and genomic SSR markers in spotted mandarin fish (Siniperca scherzeri Steindachne)

Authors: Y.Q. Dou, X.F. Liang, M. Yang, C.X. Tian, S. He and W.J. Guo

Spotted mandarin fish (Siniperca scherzeri Steindachne) feed solely on live fry of other fish species once the fry start feeding in the wild. In the present study, 26 polymorphic transcriptome-derived simple sequence repeat (SSR) markers and 14 genomic SSR markers were developed and characterized in S. scherzeri Steindachne by combining a biotin-enrichmen.. Read More»

Genet. Mol. Res. 14(4):
2015.December.29.41
DOI:
10.4238/2015.December.29.41