Ssr
Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain
Authors: F.J. Delgado-Martinez, I. Amaya, J.F. S�¡nchez-Sevilla and M.C. Gomez-Jimenez
Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.7
- DOI:
- 10.4238/2012.April.10.7
Characterization of ESTs from black locust for gene discovery and marker development
Authors: J.X. Wang, C. Lu, C.Q. Yuan, B.B. Cui, Q.D. Qiu, P. Sun, R.Y. Hu, D.C. Wu, Y.H. Sun and Y. Li
Black locust (Robinia pseudoacacia L.) is an ecologically and economically important species. However, it has relatively underdeveloped genomic resources, and this limits gene discovery and marker-assisted selective breeding. In the present study, we obtained large-scale transcriptome data using a next-generation sequencing pl.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.12
- DOI:
- 10.4238/2015.October.19.12
Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers
Authors: G.A. Luz, S.O. Gomes, R.B. Araujo Neto, M.S.C.B. Nascimento and P.S.C. Lima
Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid r.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.12
- DOI:
- 10.4238/2015.November.25.12
Genetic dissimilarity and selection of putative mutants of Terra Maranh�£o plantain cultivar using the Ward-MLM strategy
Authors: R.V. Reis, E.P. Amorim, V.B.O. Amorim, C.F. Ferreira, R.K.N. Pestana, C.A.S. Ledo, Z. Gon�§alves and A. Bor�©m
The objective of this study was to evaluate genetic variability and select putative mutants of Terra Maranhão plantain cultivar (AAB genome) subjected to gamma radiation based on agronomic data and inter simple sequence repeat molecular marker profiles using the Ward-MLM strategy. A total of 233 irradiated plants and 41.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.11
- DOI:
- 10.4238/2015.November.30.11
Natural hybridization between Gossypium mustelinum and exotic allotetraploid cotton species
Authors: I.P.P. de Menezes1, J.O. da Silva1, G. Malafaia1, R.D.D. Silveira1 andP.A.V. Barroso2
Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridiza.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.40
- DOI:
- 10.4238/2015.October.29.40
Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing
Authors: D.-A. Fang1,2, Y.-F. Zhou2, J.-R. Duan2, M.-Y. Zhang2, D.-P. Xu2, K. Liu2, P. Xu1,2* and Q. Wei3*
RNA-Seq technology has been widely applied to transcriptomics, genomics, molecular marker development, and functional gene studies. In the genome, microsatellites are simple sequence repeats (SSR) with a high degree of polymorphism that are used as DNA markers in many molecular genetic studies. Using traditional methods such as magnetic bead enr.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.1
- DOI:
- 10.4238/2015.November.13.1
Single- and double-SSR primer combined analyses in rice
Authors: J. Ma, S.C. Guan, Z. Zhang and P.W. Wang
Polymerase chain reaction (PCR) is the foundation of SSR molecular marker technology. We used sib rice varieties J518, XD1 and SD23 as experimental materials, selecting 30 pairs of SSR primers, including RM127, RM337 and RM5172, covering the rice genome, and performed single- and double-SSR primer combined analyses. We found t.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.1
- DOI:
- 10.4238/2012.April.27.1
Comparison of gSSR and EST-SSR markers for analyzing genetic variability among tomato cultivars (Solanum lycopersicum L.)
Authors: R. Zhou, Z. Wu, F.L. Jiang and M. Liang
In order to study genetic variability and develop better strategies for the utilization of 48 tomato cultivars from America, China, the Netherlands, and Portugal, genomic simple sequence repeat (gSSR) and EST-derived SSR (EST-SSR) markers were applied. In all, 15 of 82 gSSR and 18 of 115 EST-SSR markers showed polymorphic loci. There were 995 and 2072 cle.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.26.14
- DOI:
- 10.4238/2015.October.26.14
Changes on microsatellites of expressed sequence tag of sugarcane (Saccharum spp) during vegetative propagation
Authors: R. Augusto, R.C. Maranho, C.A. Mangolin, J.C. Bespalhok Filho and M.F.P.S. Machado
The reduction in sugarcane productivity in subsequent cutting stages may be related to a gradual decrease of the allele number and mean observed heterozygosity (HO) in the sugarcane ratoon. This hypothesis was tested assessing the number of alleles and HO values in 10 expressed sequence tag microsatellites (Est-SSR loci) of th.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019519
- DOI:
- 10.4238/gmr16019519
Establishment and application of molecular ID in the main inbred lines of Chinese cabbage
Authors: X.X. Zhang, X.B. Tang, Y. Liu and Y.W. Zhang
Chinese cabbage is an important cruciferous vegetable in China. The differences in the morphology and other characteristics of the different varieties of Chinese cabbage are generally caused by their different genes. Using the simple sequence repeat (SSR) DNA molecular markers is an effective way to identify different genotype.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019144
- DOI:
- 10.4238/gmr16019144