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Ssr

Cytogenomics   Research Article

Genetic variability of watermelon accessions based on microsatellite markers

Authors: R.N.C. de S. Gama C.A.F. Santos R. de C.S. Dias

We analyzed the genetic variability of 40 watermelon accessions collected from 8 regions of Northeastern Brazil using microsatellite markers, in order to suggest strategies of conservation and utilization of genetic variability in this species. These accessions are not commercial cultivars. They were sampled in areas of traditional farmers that usually keep .. Read More»

Genet. Mol. Res. 12(1):
2013.March.13.3
DOI:
10.4238/2013.March.13.3
Plant Genetics   Research Article

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Authors: J. Kumchai Y.-C. Wei C.-Y. Lee F.-C. Chen S.-W. Chin

Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them.. Read More»

Genet. Mol. Res. 12(1):
2013.March.13.4
DOI:
10.4238/2013.March.13.4
Plant Genetics   Research Article

Analysis of allopolyploidy-induced rapid genetic and epigenetic changes and their relationship in wheat

Authors: T. Qiu, Y.Z. Dong, X.M. Yu, N. Zhao and Y.F. Yang

We used the conventional and methylation-sensitive randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses to assess genome-wide changes and explore the relationships between genetic and epigenetic variations among individuals of a newly synthesized allohexaploid wheat line whose genomic cons.. Read More»

Genet. Mol. Res. 16(2):
gmr16029303
DOI:
10.4238/gmr16029303
Plant Genetics   Research Article

Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb

Authors: W.H. Zheng, Y. Zhuo, L. Liang, W.Y. Ding, L.Y. Liang and X.F. Wang

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.8.3
DOI:
http://dx.doi.org/10.4238/2015.September.8.3
Plant Genetics   Research Article

Genetic diversity and relationships among different tomato varieties revealed by EST-SSR markers

Authors: N.K. Korir, W. Diao, R. Tao, X. Li, E. Kayesh, A. Li, W. Zhen and S. Wang

The genetic diversity and relationship of 42 tomato varieties sourced from different geographic regions was examined with EST-SSR markers. The genetic diversity was between 0.18 and 0.77, with a mean of 0.49; the polymorphic information content ranged from 0.17 to 0.74, with a mean of 0.45. This indicates a fairly high degree .. Read More»

Genet. Mol. Res. 13(1):
2014.January.8.3
DOI:
10.4238/2014.January.8.3
Human Genetics   Research Article

Discovery and characterization of new microsatellite loci in Dipteryx alata Vogel (Fabaceae) using next-generation sequencing data

Authors: R.A. Guimar�£es, M.P.C. Telles, A.M. Antunes, K.M. Corr�ªa,C.V.G. Ribeiro, A.S.G. Coelho and T.N. Soares

The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered .. Read More»

Genet. Mol. Res. 16(2):
gmr16029639
DOI:
10.4238/gmr16029639
Plant Genetics   Research Article

Analysis of genetic diversity of Leuciscus leuciscus baicalensis using novel microsatellite markers with cross-species transferability

Authors: D.J. Lei, G. Zhao, P. Xie, Y. Li, H. Yuan, M. Zou, J.G. Niu and X.F. Ma

We used next-generation sequencing technology to characterize 19 genomic simple sequence repeat (SSR) markers and 11 expressed sequence tag (EST) SSR markers from Leuciscus leuciscus baicalensis, a small freshwater fish that is widely distributed in Xinjiang, China. Primers were used to test for polymorphisms in three L. leuci.. Read More»

Genet. Mol. Res. 16(2):
gmr16029376
DOI:
10.4238/gmr16029376
Plant Genetics   Research Article

Novel and highly informative Capsicum SSR markers and their cross-species transferability

Authors: G.S.C. Buso, A.M.M. Reis, Z.P.S. Amaral and M.E. Ferreira

This study was undertaken primarily to develop new simple sequence repeat (SSR) markers for Capsicum. As part of this project aimed at broadening the use of molecular tools in Capsicum breeding, two genomic libraries enriched for AG/TC repeat sequences were constructed for Capsicum annuum. A total of 475 DNA clones were sequenced from both libraries and 1.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038689
DOI:
10.4238/gmr.15038689
Plant Genetics   Research Article

Microsatellite loci in the tetraploid spined loach, Cobitis biwae, and cross-species amplification in four related species

Authors: O. Jablonska, A. Mar�­n, K. Kowalewska, T. Fujimoto and K. Arai

Fifteen microsatellite loci were identified in the tetraploid spined loach, Cobitis biwae (Teleostei: Cobitidae). Among these, 14 were polymorphic (5-31 alleles) and showed moderate to high cross-species amplification transferability in four related species, Cobitis matsubarai, Cobitis taenia, Misgurnus anguillicaudatus, and Misgurnus fossilis. The loci, .. Read More»

Genet. Mol. Res. 15(3):
gmr.15039027
DOI:
10.4238/gmr.15039027
Plant Genetics   Research Article

Comparison of RAPD and ISSR markers for assessment of genetic diversity among endangered rare Dalbergia oliveri (Fabaceae) genotypes in Vietnam

Authors: D.T. Phong, V.T.T. Hien, T.T.V. Thanh and D.V. Tang

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic.. Read More»

Genet. Mol. Res. 10(4):
2011.October.6.3
DOI:
10.4238/2011.October.6.3