Authors: R.N.C. de S. Gama C.A.F. Santos R. de C.S. Dias
We analyzed the genetic variability of 40 watermelon accessions collected from 8 regions of Northeastern Brazil using microsatellite markers, in order to suggest strategies of conservation and utilization of genetic variability in this species. These accessions are not commercial cultivars. They were sampled in areas of traditional farmers that usually keep .. Read More»
Authors: J. Kumchai Y.-C. Wei C.-Y. Lee F.-C. Chen S.-W. Chin
Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them.. Read More»
Authors: T. Qiu, Y.Z. Dong, X.M. Yu, N. Zhao and Y.F. Yang
We used the conventional and methylation-sensitive randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses to assess genome-wide changes and explore the relationships between genetic and epigenetic variations among individuals of a newly synthesized allohexaploid wheat line whose genomic cons.. Read More»
Authors: W.H. Zheng, Y. Zhuo, L. Liang, W.Y. Ding, L.Y. Liang and X.F. Wang
Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five.. Read More»
Authors: N.K. Korir, W. Diao, R. Tao, X. Li, E. Kayesh, A. Li, W. Zhen and S. Wang
The genetic diversity and relationship of 42 tomato varieties sourced from different geographic regions was examined with EST-SSR markers. The genetic diversity was between 0.18 and 0.77, with a mean of 0.49; the polymorphic information content ranged from 0.17 to 0.74, with a mean of 0.45. This indicates a fairly high degree .. Read More»
Authors: R.A. Guimar�£es, M.P.C. Telles, A.M. Antunes, K.M. Corr�ªa,C.V.G. Ribeiro, A.S.G. Coelho and T.N. Soares
The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered .. Read More»
Authors: D.J. Lei, G. Zhao, P. Xie, Y. Li, H. Yuan, M. Zou, J.G. Niu and X.F. Ma
We used next-generation sequencing technology to characterize 19 genomic simple sequence repeat (SSR) markers and 11 expressed sequence tag (EST) SSR markers from Leuciscus leuciscus baicalensis, a small freshwater fish that is widely distributed in Xinjiang, China. Primers were used to test for polymorphisms in three L. leuci.. Read More»
Authors: G.S.C. Buso, A.M.M. Reis, Z.P.S. Amaral and M.E. Ferreira
This study was undertaken primarily to develop new simple sequence repeat (SSR) markers for Capsicum. As part of this project aimed at broadening the use of molecular tools in Capsicum breeding, two genomic libraries enriched for AG/TC repeat sequences were constructed for Capsicum annuum. A total of 475 DNA clones were sequenced from both libraries and 1.. Read More»
Authors: O. Jablonska, A. MarÃ?Ân, K. Kowalewska, T. Fujimoto and K. Arai
Fifteen microsatellite loci were identified in the tetraploid spined loach, Cobitis biwae (Teleostei: Cobitidae). Among these, 14 were polymorphic (5-31 alleles) and showed moderate to high cross-species amplification transferability in four related species, Cobitis matsubarai, Cobitis taenia, Misgurnus anguillicaudatus, and Misgurnus fossilis. The loci, .. Read More»
Authors: D.T. Phong, V.T.T. Hien, T.T.V. Thanh and D.V. Tang
Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic.. Read More»