Transcriptase
Combination of multiple gene markers to detect circulating tumor cells in the peripheral blood of patients with non-small cell lung cancer using real-time PCR
Authors: M. Ding, X. Li and T. Qiu
Our study aims to determine the clinical significance of human telomerase reverse transcriptase (hTERT), S-phase kinase-associated protein 2 (Skp2) and thyroid transcription factor-1 (TTF-1) mRNA expressions in peripheral blood (PB) of patients with non-small cell lung cancer (NSCLC). Real-time polymerase chain reaction was used to investigate the gene ex.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.24
- DOI:
- 10.4238/2015.October.21.24
shRNA inhibits the expression of chicken telomerase reverse transcriptase in MDCC-MSB1 cells
Authors: N. Jiang, X.B. Zheng, Z.Y. Zhao, Z.G. Qin and T.J. Liu
Here, we investigated the effects of blocking chicken telomerase reverse transcriptase (chTERT) in MDCC-MSB1 cells, using small-hairpin RNAs (shRNAs) to interfere with gene expression. shRNAs specific to chTERT mRNA were designed, cloned into DNA plasmid vectors, and transfected into MDCC-MSB1 cells. The transfected chTERT RNAs were expressed by the RNA p.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016979
- DOI:
- 10.4238/gmr.15016979
Molecular characterization of an expressed sequence tag representing the cuticle-degrading serine protease gene (PII) from the nematophagous fungus Arthrobotrys oviformis by differential display technology
Authors: A. Nagee, A. Acharya, A. Shete, P.N. Mukhopadhyaya and B.A. Aich
The technology of mRNA-based differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR) was used to detect a 246-bp differentially expressed fragment from the nematophagous fungus Arthrobotrys oviformis when young mycelia were induced with the round worm Haemonchus contortus. The fragment was converted into an expressed sequence tag (.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr487
- DOI:
- 10.4238/vol7-4gmr487
Molecular and chromosomal mutations among children with B-lineage lymphoblastic leukemia in Brazilâ??s Federal District
Authors: D.R. Mesquita J.C. C?³rdoba ?.Q. Magalh?£es M.S. C?³rdoba J.R.C. Oliveira A. Gon?§alves I. Ferrari C. Martins-de-S?¡
Acute lymphoblastic leukemia (ALL) accounts for approximately 80% of all acute leukemias during childhood. Chromosomal anomalies resulting from gene fusion, which are frequent in leukemias, create hybrid transcripts, the great majority of which encode transcription factors. We analyzed 88 pediatric patients (median age 7.3 yea.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr582
- DOI:
- 10.4238/vol8-1gmr582
Heterogeneous evolution of Ty3-gypsy retroelements among bamboo species
Authors: M.M. An, C. Guo, P.P. Lin and M.B. Zhou
Ty3-gypsy long-terminal repeat retroelements are ubiquitously found in many plant genomes. This study reports the occurrence of heterogeneous Ty3-gypsy retroelements in four representative bamboo species: Phyllostachys heterocycla (Carr.) Mitford cv. pubescens, P. heterocycla (Carr.) Mitford cv. heterocycla, Dendrocalamopsis oldhami, and Pleioblastus fort.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038515
- DOI:
- 10.4238/gmr.15038515