Transformation
Ectopic Expression of AtCIPK23 Enhances Drought Tolerance via Accumulating Less H2O2 in Transgenic Tobacco Plants
Authors: Liming Lu, Linglong Yang, Ke Li, Yifei Lu, Liqin Li
Drought is a major threat to agricultural crop growth and productivity. However, the molecular mechanism behind such effects remains largely unknown. Plant CBL (Calcineurin B-Like)-interacting protein kinases (CIPKs) are believed to play an important role in plant drought tolerance and signalling transduction. Here, we report .. Read More»
- Genet. Mol. Res. 17(1):
- gmr16039864
- DOI:
- 10.4238/gmr16039864
Optimization of a biolistic transformation system for transfer of antifreeze gene KN2 and the bar herbicide resistance gene in common wheat
Authors: L. Cai, D.F. Sun and G.L. Sun
We studied the effects of different media for callus induction and differentiation, and pre-culture period of immature wheat embryo culture on biolistic transformation efficiency for including antifreeze gene KN2 and bar conferring resistance to the herbicide bialaphos. The percentage of plantlets generated from induction and .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.8
Efficient production of transgenic melon via Agrobacterium-mediated transformation
Authors: I. Bezirganoglu, S.Y. Hwang, J.F. Shaw and T.J. Fang
Oriental melon (Cucumis melo L. var. makuwa) is an important fruit for human consumption. However, this plant species is one of the most recalcitrant to genetic transformation. The lack of an efficient in vitro system limits the development of a reproducible genetic transformation protocol for Oriental melon. In this study, an.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.7
- DOI:
- 10.4238/2014.April.25.7
Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants
Authors: C.R. Muniz, G.F. da Silva, M.T. Souza Jr., F.C.O. Freire, G.H.J. Kema and M.I.F. Guedes
Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotra.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.21.8
- DOI:
- 10.4238/2014.February.21.8
Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers
Authors: J.B. Qin, Y. Wang and C.Q. Zhu
Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella .. Read More»
- Genet. Mol. Res. 13(2):
Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone
Authors: R. Liu, H.H. Zhang, Z.X. Chen, M.Q. Shahid, X.L. Fu and X.D. Liu
Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.29
- DOI:
- 10.4238/2015.October.28.29
Characterization of a rice germin-like protein gene promoter
Authors: T. Mahmood T. Yasmin M.I. Haque S.M.S. Naqvi
Germin and germin-like proteins (GLPs) are water-soluble extracellular proteins that are expressed in response to specific environmental and developmental signals. Although some enzymatic activities have also been associated with germin/GLPs, their role in overall metabolism is not fully understood. However, insight into their function may be gained by an.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.6
- DOI:
- 10.4238/2013.February.7.6
Construction and transformation of expression vector containing Panax japonicus SS gene
Authors: L. Zhang and T.T. Wang
Panax japonicus C.A. Meyer, a perennial herb belonging to the Araliaceae ginseng genus, is one of the seven rare and endangered Chinese medical herbs. By cloning the SS segment, the expression vectors pCXSN-PjSS and pCXSN-antiPjSS were constructed and introduced into Agrobactria LBA4404, which is used for engineering bacteria... Read More»
- Genet. Mol. Res. 16(1):
- gmr16019341
- DOI:
- 10.4238/gmr16019341
Overexpression of the CmACS-3 gene in melon causes abnormal pollen development
Authors: H. Zhang and F. Luan
Sexual diversity expressed by the Curcurbitaceae family is a primary example of developmental plasticity in plants. Most melon genotypes are andromonoecious, where an initial phase of male flowers is followed by a mixture of bisexual and male flowers. Over-expression of the CmACS-3 gene in melon plants showed an increased number of flower buds, and increa.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.8.4
- DOI:
- http://dx.doi.org/10.4238/2015.September.8.4
An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.)
Authors: H.J. Zhang, P. Gao, X.Z. Wang and F.S. Luan
An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation, using cotyledon node zone-stem connection region of melon, has been developed. The new Agrobacterium-mediated transformation methodology, independent of organ culture, used the entire germinated seed as explants. The transformatio.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.8.4
- DOI:
- 10.4238/2014.January.8.4