Transformation
Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues
Authors: X.G. Han, D.K. Wang, F. Gao, R.H. Liu and Z.G. Bi
Bone morphogenetic protein 2 (BMP-2) can promote fracture healing. Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear. Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis. In this study, tissue composition and express.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.21.19
- DOI:
- http://dx.doi.org/10.4238/2015.September.21.19
Cloning and characterization of the UBC gene from lotus (Nelumbo nucifera)
Authors: Y. Diao, G.L. Li, A.Q. Yu, X.W. Zheng, K.Q. Xie, Y.W. Wang, M.Q. Zhou, J. Ming and Z.L. Hu
Protein ubiquitination is extensively involved in the regulation of a considerable number of physiological processes in plant cells. E2 (ubiquitin-conjugating enzyme, UBC), one of the essential enzymes of eukaryotic ubiquitination, catalyzes protein ubiquitination together with E1 and E3. In this study, we cloned four full-length cDNANnUBCs of Nelumbo nuc.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038341
- DOI:
- 10.4238/gmr.15038341
Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.) using the expansin 10 (CsEXP10) gene
Authors: Y.D. Sun, W.R. Luo, S.Y. Sun and L. Ni
The cucumber expansin 10 (CsEXP10) gene was previously cloned from young cucumber fruits but its role has not been defined. To determine the role of this gene in plant growth and development, a CsEXP10 gene transformation system was established. The open reading frame of the gene was inserted behind the CaMV35S promoter of vector pCAMBIA1301, and the cons.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Characterization of chloroplast region rrn16-rrn23S from the tropical timber tree Cedrela odorata L. and de novo construction of a transplastomic expression vector suitable for Meliaceae trees and other economically important crops
Authors: L.A. LÃ?³pez-Ochoa, M.M. Apolinar-HernÃ?¡ndez and Y.J. PeÃ?±a-RamÃ?Ârez
The forest tree Spanish cedar (Cedrela odorata L.) is well-known for its high-value timber; however, this species is attacked by the shoot borer (Hypsipyla grandella) during its early years of development, resulting in branched stems and making the plants useless for high-quality wood production. The generation of resistant varieties expressing entomotoxi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.2
- DOI:
- 10.4238/2015.February.20.2
Characterization of the �²-1,3-glucanase gene in peanut (Arachis hypogaea L.) by cloning and genetic transformation
Authors: L.X. Qiao, X. Ding, H.C. Wang, J.M. Sui and J.-S. Wang
Plant b-1,3-glucanases are commonly involved in disease resistance. This report describes the cloning and genetic transformation of a b-1,3-glucanase gene from peanut. The gene was isolated from both the genomic DNA and cDNA of peanut variety Huayu20 by polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), re.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.17
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.17
Improved production of transgenic Dioscorea zingiberensis (Dioscoreaceae) by Agrobacterium tumefaciens-mediated transformation
Authors: L. Shi, J.Q. Fan, C.G. Hu, J. Luo and J.L. Yao
The establishment of high-efficiency Agrobacterium-mediated transformation techniques could improve the production of Dioscorea zingiberensis, a medicinal species with a high diosgenin content. We co-cultivated embryogenic calli induced from mature seeds with A. tumefaciens strain EHA105. A binary vector, pCAMBIA1381, which co.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.3.4
- DOI:
- 10.4238/2012.February.3.4
Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages
Authors: M.Q. Zhang, C.A. Ma, W.W. Lv, Y.H. Huang, D.L. Wang and Y.L. Zhao
To better understand the reproductive transformation mechanism of Daphnia carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, and 85.11% identity to orthologous genes in .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.10
- DOI:
- 10.4238/2015.June.1.10
Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21
Authors: L. Liu, Y.M. Wei, X.W. Zhou, J. Lin, X.F. Sun and K.X. Tang
An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304’AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly i.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.7
- DOI:
- 10.4238/2013.August.12.7
Improving plant transformation using Agrobacterium tumefaciens
Authors: L.V. Ribeiro Neto, A.P. Oliveira, M.V. Louren�§o, B.W. Bertoni, S.C. Fran�§a, T.M. Rosa-Santos and S.M. Zingaretti
Here, we report a quick and low-cost method to improve plant transformation using Agrobacterium tumefaciens. This method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. We show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacte.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.13
- DOI:
- 10.4238/2015.June.18.13
Construction of overexpression vectors of Magnaporthe oryzae genes BAS1 and BAS4 fusion to mCherry and screening of overexpression strains
Authors: M.L. Liang, J.L. Yan, Y.Q. Yang, L. Liu, C.Y. Li and J. Yang
The aim of this study was to construct overexpression vectors and selecting strains of the Magnaporthe oryzae effectors BAS1 and BAS4. Primer pairs of BAS1, BAS4, and mCherry were designed based on their known nucleotide sequences. The coding sequences of BAS1 and BAS4 were amplified, and the pXY201 plasmid was selected as a template to amplify the mCherr.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.17
- DOI:
- 10.4238/2015.June.26.17