Transformation
Improved Agrobacterium-mediated transformation and high efficiency of root formation from hypocotyl meristem of spring Brassica napus â??Precocityâ?? cultivar
Authors: X.X. Liu, S.R. Lang, L.Q. Su, X. Liu and X.F. Wang
Rape seed (Brassica napus L.) is one of the most important oil seed crops in the world. Genetic manipulation of rapeseed requires a suitable tissue culture system and an efficient method for plant regeneration, as well as an efficient transformation procedure. However, development of transgenic B. napus has been problematic, and current studies are limite.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.11
- DOI:
- 10.4238/2015.December.14.11
A minimal DNA cassette as a vector for genetic transformation of soybean (Glycine max)
Authors: G.R. Vianna, F.J.L. Arag?£o and E.L. Rech
Currently, the market demands products committed to protecting human health and the environment, known as clean products. We developed a protocol using DNA fragments containing only the gene sequence of interest, to replace the circular vectors containing genes for antibiotic resistance and other undesirable sequences, for obt.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1058
- DOI:
- 10.4238/vol10-1gmr1058
Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots
Authors: W.C. Li, S.D. Ji, X.C. Wang, Z.K. Li, H.C. Zhang, C.Z. Tian,Y.L. Liuand C.X. Duan
Corn DNA was introduced into dry seeds of rice (cv. ‘YuJing-6’) by ion beam irradiation. Proteinase activities in rice seedling roots were subsequently analyzed by renaturation electrophoresis at pH 4.5, 7.0, and 8.5. Proteinase activity was more pronounced on gels at higher pH. Irradiation of r.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.29.19
- DOI:
- 10.4238/2015.June.29.19
Easy detection of green fluorescent proteinmulticopy transformants in Penicillium griseoroseum
Authors: Francis J.F. Lopes, Elza F. de Ara?ºjo and Marisa V. de Queiroz
Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the p.. Read More»
- Genet. Mol. Res. 3(4):
Oncogene-induced transcriptional patterns in established cell lines as a model for in vitro analysis of tumor biology
Authors: Francisco de Paula Careta, Bruno Marcos Verbeno Azevedo,Beatriz Alves Vianna1, Greiciane Gaburro Paneto,Irineu Iester Degasperi, Marcos do Carmo Oyama,Val?©ria Fagundes, ??ngela Maria Spagnol Perrone andI?ºri Drumond Louro
Established cell lines have long been used for in vitro studies of tumor biology, enabling investigators to control growth conditions and to draw important conclusions about the oncogenic microenvironment. However, gene expression behavior in cultured cells may not always reflect the actual in vivo scenario, and analysis deriv.. Read More»
- Genet. Mol. Res. 3(3):
Genetic transformation of the lettuce cultivar Grand Rapids (Lactuca sativa L.) by Agrobacterium tumefaciens to improve osmotic stress tolerance (Transforma?§?£o gen?©tica de alface (Lactuca sativa L.), cultivar â??Grand Rapidsâ?, via Agrobacterium tumefaciens, para melhoramento de toler?¢ncia ao estresse h?drico)
Authors: Marcos Pileggi
-.. Read More»
- Genet. Mol. Res. 1(2):