Cytogenomics
Cytotoxicity of 1-dodecyl-3-methylimidazolium bromide on HepG2 cells
Authors: T.H. Li, C.Q. Jing, K.L. Gao, W.Y. Yue and S.F. Li
We evaluated the cytotoxicity of 1-dodecyl-3-methylimidazolium bromide ([C12mim][Br]) on HepG2 cells and its influence on plasma membrane permeability. The results showed that [C12mim][Br] inhibited HepG2 cell growth and decreased cell viability in a concentration-dependent manner. The results also revealed that [C12mim][Br] exposure induced apoptosis in .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.26.31
- DOI:
- 10.4238/2015.October.26.31
cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1
Authors: W.-R. Hou Y.-L. Hou X. Ding T. Wang
The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.3.5
- DOI:
- 10.4238/2012.September.3.5
Bottom-up assembly of RNA nanoparticles containing phi29 motor pRNA to silence the asthma STAT5b gene
Authors: C. Qiu W.K. Peng F. Shi T. Zhang
Activation of the transcription factor signal transducer and activator of transcription 5b (STAT5b) is a key event in the development of asthma. The potent ability of small interfering RNA (siRNA) to inhibit the expression of STAT5b mRNA has provided a new class of therapeutics for asthma. However, efficient delivery of siRNAs remains a key obstacle to th.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.12.6
- DOI:
- 10.4238/2012.September.12.6
Variable expressivity of osteogenesis imperfecta in a Brazilian family due to p.G1079S mutation in the COL1A1 gene
Authors: M.V.D. Moraes M. Milanez B.V.P. Almada V. Sipolatti M.R.G.O. Rebou�?§as V.R.R. Nunes A.N. Akel Jr. M. Zatz F.I.V. Errera I.D. Louro F. Paula
Osteogenesis imperfecta (OI) is a Mendelian disease with genetic heterogeneity characterized by bone fragility, recurrent fractures, blue sclerae, and short stature, caused mostly by mutations in COL1A1 or COL1A2 genes, which encode the pro-α1(I) and pro-α2(I) chains of type I collagen, respectively. A Brazilian family that showed variable exp.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.12.7
- DOI:
- 10.4238/2012.September.12.7
Role of mitochondrial DNA variants and copy number in diabetic atherogenesis
Authors: M.C. Chien W.T. Huang P.W. Wang C.W. Liou T.K. Lin C.J. Hsieh S.W. Weng
Hyperglycemia-induced reactive oxygen species production can cause diabetes and its complications, including atherosclerosis. The role of mitochondrial DNA variants and mitochondrial copy number in the pathogenesis of diabetic atherogenesis is not well understood. We examined 36 diabetic patients who had undergone amputation for diabetic foot and seven non-d.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.17.4
- DOI:
- 10.4238/2012.September.17.4
Nucleotide variation in the mitochondrial genome provides evidence for dual routes of postglacial recolonization and genetic recombination in the northeastern brook trout (Salvelinus fontinalis)
Authors: B.L. Pilgrim R.C. Perry J.L. Barron H.D. Marshall
Levels and patterns of mitochondrial DNA (mtDNA) variation were examined to investigate the population structure and possible routes of postglacial recolonization of the worldâ�?�?s northernmost native populations of brook trout (Salvelinus fontinalis), which are found in Labrador, Canada. We analyzed the sequence diversity of a 1960-bp portion of the mitoc.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.26.2
- DOI:
- 10.4238/2012.September.26.2
Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers
Authors: Z. Noormohammadi Y. Hasheminejad-Ahangarani Farahani M. Sheidai S. Ghasemzadeh-Baraki O. Alishah
Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.30.12
- DOI:
- 10.4238/2013.January.30.12
SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation
Authors: C.L. Deng R.Y. Qin J. Gao Y.Y. Jia Y.X. Ren W.J. Gao L.D. Lu
Ion implantation, a new biophysically mutagenic technique, has shown great potential for crop breeding. To reveal the mutation effect of low-energy ion implantation on Baiyangdian red lotus, sequence-related amplified polymorphism markers were used to amplify and detect the DNA sequence differences in mutants induced by Fe+ ion implantation. A total of 12.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.3
- DOI:
- 10.4238/2013.February.7.3
Characterization of a rice germin-like protein gene promoter
Authors: T. Mahmood T. Yasmin M.I. Haque S.M.S. Naqvi
Germin and germin-like proteins (GLPs) are water-soluble extracellular proteins that are expressed in response to specific environmental and developmental signals. Although some enzymatic activities have also been associated with germin/GLPs, their role in overall metabolism is not fully understood. However, insight into their function may be gained by an.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.6
- DOI:
- 10.4238/2013.February.7.6
Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
Authors: G.B. Nogueira M.V. Queiroz R.A. Ribeiro E.F. Ara�?ºjo
Colletotrichum lindemuthianum is the causal agent of plant bean anthracnose, one of the most important diseases affecting the common bean. We investigated the structure and expression of the nit1 gene (nitrate reductase) of C. lindemuthianum. The nit1 gene open reading frame contains 2787 bp, interrupted by a single 69-bp intron. The predicted protein has 90.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.8.7
- DOI:
- 10.4238/2013.February.8.7