Plant Genetics
A priori choice of hybrid parents in plants
Authors: Luiz Ant?´nio dos Santos Dias, Edgard Augusto de Toledo Picoli, Rodrigo Barros Rocha and Acelino Couto Alfenas
Plant breeding deals with high-yielding genotypes. However, how best to choose parents of these genotypes remains an unsolved question. Here, we focus on a priori choice based on parental distances by means of agronomic and molecular data. Despite numerous theoretical and empirical studies, a priori choice continues to be a co.. Read More»
- Genet. Mol. Res. 3(3):
Putative pyrophosphate phosphofructose 1-kinase genes identified in sugar cane may be getting energy from pyrophosphate
Authors: S.J. Suzuki, M.A. Mutton, J.M. Pizauro, M. I.T. Ferro, M. V.F. Lemos, M. J.R. Mutton, S. M.Z. Di Mauro
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have identified both the α and β subunits of this e.. Read More»
- Genet. Mol. Res. 2(4):
Molecular characterization of ABC transporter-encoding genes in Aspergillus nidulans
Authors: Adriana Mendes do Nascimento, Maria Helena S. Goldman, Gustavo H. Goldman
As a preliminary step towards characterizing genes encoding ATP-binding cassette (ABC) transporters that confer pleiotropic drug resistance in Aspergillus, we used a PCR-based approach to isolate four DNA fragments corresponding to different ABC type transporter genes. DNA sequencing and Southern blot analysis confirmed that t.. Read More»
- Genet. Mol. Res. 1(4):
Cassava, Manihot esculenta Crantz, genetic resources: origin of the crop, its evolution and relationships with wild relatives
Authors: N.M.A. Nassar
About 98 species of Manihot are known. All of them are native to the New World and are concentrated in four regions in Brazil and Central America. All the Manihot species so far examined have 2n = 36 chromosomes. Interspecific hybrids between cassava and its wild relatives show relatively normal meiosis, and further generation.. Read More»
- Genet. Mol. Res. 1(4):
Inheritance of resistance to Meloidogyne incognita race 2 in the hot pepper cultivar Carolina Cayenne(Capsicum annuum L.)
Authors: Fausto de Souza-Sobrinho, Wilson Roberto Maluf, Luiz A.A. Gomes and Vicente Paulo Campos
Root-knot nematodes of the genus Meloidogyne are important pathogens affecting vegetable crop production in Brazil and worldwide. The pepper species Capsicum annuum includes both hot and sweet peppers; very little emphasis has been placed on breeding sweet peppers for nematode resistance. We report on the inheritance of resist.. Read More»
- Genet. Mol. Res. 1(3):
Greater genetic variability in Argentine Creole than in Thoroughbred horses based on serum protein polymorphisms
Authors: S. D?az, F.N. Dulout and P. Peral-Garc?a
Genetic polymorphism was analyzed for five blood proteins: albumin - Al, esterase - Es, α1B-glycoprotein - Xk, transferrin - Tf and hemoglobin - Hb in 200 Thoroughbred (TB) and 124 Argentine Creole (AC) horses. Of the five systems examined, Tf and Hb were not in Hardy-Weinberg equilibrium in either breed and Es was not i.. Read More»
- Genet. Mol. Res. 1(3):
CENP-B is a conserved gene among vegetal
Authors: Olga Barbosa-Cisneros and Rafael Herrera-Esparza
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a .. Read More»
- Genet. Mol. Res. 1(3):
Genetic transformation of the lettuce cultivar Grand Rapids (Lactuca sativa L.) by Agrobacterium tumefaciens to improve osmotic stress tolerance (Transforma?§?£o gen?©tica de alface (Lactuca sativa L.), cultivar â??Grand Rapidsâ?, via Agrobacterium tumefaciens, para melhoramento de toler?¢ncia ao estresse h?drico)
Authors: Marcos Pileggi
-.. Read More»
- Genet. Mol. Res. 1(2):
A model system for testing gene vectors using murine tumor cells on the chorioallantoic membrane of the chick embryo
Authors: Sergio U. Dani and Rachel Espindola
We developed a model system for testing gene vectors, based on the growth of murine tumors on the chorioallantoic membrane (CAM) of embryonic chickens. The ability of selected murine cells to grow on the CAM was rated according to the following criteria: i) formation of tumor masses; ii) metastasis formation; iii) reproducibil.. Read More»
- Genet. Mol. Res. 1(2):
Apomixis and cassava
Authors: N.M.A. Nassar
Apomixis means seed formation without fertilization. In cassava (Manihot esculenta) it is an alternative to reproduction by cuttings, which normally transmits pathogens and leads to an accumulation of viral and bacterial diseases. Apomixis also assures preservation of heterosis and avoids genetic segregation. It occurs in wild.. Read More»
- Genet. Mol. Res. 1(2):